Identification of Epinephelus malabaricus and Epinephelus coioides using DNA markers

1 IntroductionBothEpinephelus malabaricusandE. coioidesbelong to the genusEpinephelus(Perciformes: Ser-ranidae: Epinephelinae).They are warm water reeffishes that have similar distribution ranges as well assimilar morphological characteristics, which have…     

Effects of an Artificial Breakwater on the Distributions of Planktonic Microbial Communities

The summer distributions of planktonic microbial communities (heterotrophic and phtosynthetic bacteria, phtosynthetic and heterotrophic nanoflagellates, ciliate plankton, and microphytoplankton) were compared between inner and outer areas of Lake Sihwa, divided by an artificial breakwater, located on the western coast of Korea, in September 2003. The semienclosed, inner area was characterized by hyposaline surface water (<17 psu), and by low concentrations of dissolved oxygen (avg. 0.4 mg L¹) and high concentrations of inorganic nutrients (nitrogenous nutrients >36 μM, phosphate <4 μM) in the bottom layer. Higher densities of heterotrophic bacteria and nanoflagellates also occurred in the inner area than did in the outer area, while microphytoplankton (mainly diatoms) occurred abundantly in the outer area. A tiny tintinnid ciliate, Tintinnopsis nana, bloomed into more than 10⁶ cells L¹ at the surface layer of the inner area, while its abundance was much lower (10³-10⁴ cells L¹) in the outer area of the breakwater. Ciliate abundance was highly correlated with heterotrophic bacteria (r = 0.886, p < 0.001) and heterotrophic flagellates (r = 0.962, p < 0.001), indicating that rich food availability may have led to theT. nana bloom. These results suggest that the breakwater causes the eutrophic environment in artificial lakes with limited flushing of enriched water and develops into abundant bacteria, nanoflagellates, and ciliates.     

山溪性可冲性强潮河口曹娥江高水位初步研究

应用多年的实测资料 ,分析了曹娥江河口高水位成因 ,结果表明 ,曹娥江河口洪水位具有山溪性和可冲性的特点 ,人类活动对高水位有较大影响。应用统计分析法和成因分析法推求 1 %设计高水位 ,讨论了成因分析法中上、下边界条件的选取 ,其选取原则对其他潮汐河口确定设计高水位具有借鉴意义     

On dynamic coupling effects between a spar and its mooring lines

The responses of a spar constrained by slack mooring lines to steep ocean waves and tensions in the mooring lines are simulated using two different numerical schemes: a quasi-static approach (SMACOS) and a coupled dynamic approach (COUPLE). The two approaches are the same in computing wave loads on the structure. Their difference is in modeling dynamic forces of mooring lines; that is the dynamic forces are included in the computation of COUPLE but neglected in SMACOS. The numerical simulation is examined against the laboratory measurements of the JIP Spar in a water depth of 318 m. The dynamic coupling effects between the JIP Spar and its mooring lines in different water depths (318, 618 and 1018 m) are investigated by the comparison of numerical simulations obtained using the quasi-static and coupled dynamic approaches. It is found that the damping of mooring lines reduces the slow-drift surge and pitch of the Spar, especially in deep water. The reduction in the amplitude of slow-drift surge can reach about 10% in a water depth of 1018 m. The tension in mooring lines may greatly increase in the wave frequency range when dynamic forces in mooring lines are considered. The mooring-line tension in the wave frequency range predicted by the coupled dynamic approach can be eight times as great as the corresponding prediction by the quasi-static approach in a water depth of 1018 m. This finding may have important implications for the estimation of the fatigue strength and life span of the mooring lines deployed in deep water oceans.     

白洋淀重新蓄水后的轮虫

1989年对白洋淀重新蓄水后的轮虫作了采样调查,共采到轮虫123种,隶属于46属12科,并对鉴定到种的轮虫作了初步分析。     

Atlas of Japan (East) Sea hydrographic properties in summer, 1999

Hydrographic properties from CTD and discrete bottle sample profiles covering the Japan (East) Sea in summer, 1999, are presented in vertical sections, maps at standard depths, maps on isopycnal surfaces, and as property–property distributions. This data set covers most of the Sea with the exception of the western boundary region and northern Tatar Strait, and includes nutrients, pH, alkalinity, and chlorofluorocarbons, as well as the usual temperature, salinity, and oxygen observations.     

化学生态调控对鳌山湾氮磷营养盐的影响

鳌山湾,是一个营养类型特殊的海湾,湾中基本无径流输入,呈“C”字形,封闭性较强,水较浅,湾内主要有筏式扇贝养殖和底播贝类养殖,沿岸有滩涂池塘养殖.根据1996年11月至1998年5月的调查结果得知,鳌山湾海域为贫营养海域[1,2].为探讨贫营养海域进行化学生态调控的可能性,利用鳌山湾的封闭性,根据其水交换规律[3],于1999年6月首次对鳌山湾进行了全湾规模较大的化学生态调控试验,旨在有效提高鳌山湾的营养水平,为今后在贫营养海域合理发展海水养殖业提供科学依据,获得了十分理想的效果.     

用16S rRNA基因的内切酶图谱快速鉴别几种对虾病原菌

坎普氏弧菌是青岛海洋大学生物系微生物实验室于１９８９－１９９０年自对虾养殖场中国对虾红腿病心脏及血淋巴中分离并鉴定的菌株，副溶血菌和溶藻胶弧菌两菌株于１９９４年９月得自中国科学院微生物研究所，为建立快速、准确的中国对虾病原菌的诊断技术，根据几种细菌的１６ＳｒＲＮＡ基因的序列，设计并合成该基因的多聚酶反应的引物ＰＬ１和ＰＬ２。并用该对引物分别从坎普氏弧菌、副溶血弧菌和溶藻胶弧菌的ＤＮＡ要品中扩增出分     

几株原甲藻核糖体大亚基RNA基因的部分克隆及序列分析

对7株赤潮原甲藻28S rDNA 5'端部分序列进行扩增、克隆和序列测定,并从GeneBank上获取14个原甲藻28S rDNA序列,用NJ法和ME法构建了原甲藻属的系统树,并对序列进行分析.结果表明,7株原甲藻28S rDNA扩增序列长度为950~958 bp,通过NJ法和ME法构建的系统树完全一致.大部分分离自不同海域的同种原甲藻的序列高度保守,而不同种间在序列高变区却有较大的差异.但来自南海海域的海洋原甲藻(Prorocentrum micans)与分离自其他海域的株系序列差异较大,甚至超出了有些种间的差异.由28S rDNA高变区获得的序列,有望成为浮游植物特异性分子探针设计的良好靶区域.